Blar i forfatter "Lockwood, Glen P"

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    • Cost-efficient nanoscopy reveals nanoscale architecture of liver cells and platelets 

      Mao, Hong; Diekmann, Robin; Liang, Hai; Cogger, Victoria Carroll; Le Couteur, David George; Lockwood, Glen P; Hunt, Nick; Schuttpelz, Mark; Huser, Thomas Rolf; Chen, Vivien; McCourt, Peter Anthony (Journal article; Tidsskriftartikkel; Peer reviewed, 2019-07-09)
      Single-molecule localization microscopy (SMLM) provides a powerful toolkit to specifically resolve intracellular structures on the nanometer scale, even approaching resolution classically reserved for electron microscopy (EM). Although instruments for SMLM are technically simple to implement, researchers tend to stick to commercial microscopes for SMLM implementations. Here we report the construction ...

    • The Effects of Metformin on Age-Related Changes in the Liver Sinusoidal Endothelial Cell 

      Hunt, Nicholas J; Lockwood, Glen P; Kang, Sun; Pulpitel, Tamara; Clark, Ximonie; Mao, Hong; McCourt, Peter Anthony; Cooney, Greg; Wali, Jibran; Le Couteur, Frank; Le Couteur, David G; Cogger, Victoria C (Journal article; Tidsskriftartikkel; Peer reviewed, 2020-06-14)
      Age-related changes in the liver sinusoidal endothelium, particularly the reduction in fenestrations, contribute to insulin resistance in old age. Metformin impacts on the aging process and improves insulin resistance. Therefore, the effects of metformin on the liver sinusoidal endothelium were studied. Metformin increased fenestrations in liver sinusoidal endothelial cells isolated from both young ...

    • The effects of metformin on age-related changes in the liver sinusoidal endothelial cell 

      Hunt, Nick; Lockwood, Glen P; Kang, Sun; Pulpitel, Tamara; Clark, Ximonie; Mao, Hong; McCourt, Peter Anthony; Cooney, Greg; Wali, Jibran; Le Couteur, Frank; Le Couteur, David George; Cogger, Victoria Carroll (Journal article; Tidsskriftartikkel; Peer reviewed, 2019-06-14)
      Age-related changes in the liver sinusoidal endothelium, particularly the reduction in fenestrations, contribute to insulin resistance in old age. Metformin impacts on the aging process and improves insulin resistance. Therefore, the effects of metformin on the liver sinusoidal endothelium were studied. Metformin increased fenestrations in liver sinusoidal endothelial cells isolated from both young ...

    • Manipulating fenestrations in young and old liver sinusoidal endothelial cells 

      Hunt, Nicholas J; Lockwood, Glen P; Warren, Alessandra; Mao, Hong; McCourt, Peter Anthony; Le Couteur, David George; Cogger, Victoria Carroll (Journal article; Tidsskriftartikkel; Peer reviewed, 2018-12-27)
      Fenestrations are pores within liver sinusoidal endothelial cells (LSECs) that enable the transfer of substrates (particularly insulin and lipoproteins) between blood and hepatocytes. With increasing age, there are marked reductions in fenestrations, referred to as pseudocapillarization. Currently, fenestrations are thought to be regulated by vascular endothelial growth factor and nitric oxide (NO) ...

    • Quantum Dot Nanomedicine Formulations Dramatically Improve Pharmacological Properties and Alter Uptake Pathways of Metformin and Nicotinamide Mononucleotide in Aging Mice 

      Hunt, Nick; Lockwood, Glen P; Kang, Sun Woo; Westwood, Lara; Limantoro, Christina; Chrzanowski, Wojciech; McCourt, Peter Anthony; Kuncic, Zdenka; Le Couteur, David G; Cogger, V.C. (Journal article; Tidsskriftartikkel; Peer reviewed, 2021-02-24)
      Orally administered Ag<sub>2</sub>S quantum dots (QDs) rapidly cross the small intestine and are taken up by the liver. Metformin and nicotinamide mononucleotide (NMN) target metabolic and aging processes within the liver. This study examined the pharmacology and toxicology of QD-based nanomedicines as carriers of metformin and NMN in young and old mice, determining if their therapeutic potency and ...

    • Rapid Intestinal Uptake and Targeted Delivery to the Liver Endothelium Using Orally Administered Silver Sulfide Quantum Dots. 

      Hunt, Nicholas J; Lockwood, Glen P; Le Couteur, Frank H.; McCourt, Peter Anthony; Singla, Nidhi; Kang, Sun; Burgess, Andrew; Kuncic, Zdenka; Le Couteur, David G; Cogger, Victoria C (Journal article; Tidsskriftartikkel; Peer reviewed, 2020-01-24)
      Quantum dots (QDs) are used for imaging and transport of therapeutics. Here we demonstrate rapid absorption across the small intestine and targeted delivery of QDs with bound materials to the liver sinusoidal endothelial cells (LSECs) or hepatocytes in vitro and in vivo following oral administration. QDs were radiolabeled with <sup>3</sup> H-oleic acid, with a fluorescent tag or <sup>14</su ...